Coding

Part:BBa_K5293006

Designed by: Arnaud Boudigou   Group: iGEM24_uOttawa   (2024-09-23)


His_mScarlet_TEV_Sema

Our project utilized His-mScarlet-TEV-semaglutide as an insert in multiple vectors such as pCLGG and pHREAC. By cloning this insert into plasmid vectors for biopharming, we expressed fusion protein mScarlet-semaglutide in plant species Nicotiana benthamiana. This resulted in stable expression, and enabled the purification and quantification of our protein of interest.

Sequence and Features

DNA Sequence encoding a small peptide related to semaglutide, a GLP 1 Agonist. It is lacking post-translational modification to be semaglutide. It is fused to a histag for purification followed by a linker (6xGlycine), mScarlet as a easy tool to observer protein production & purification, another linker (6xGly) and TEV site before the Semaglutide sequence.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 826
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 826
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 826
  • 1000
    COMPATIBLE WITH RFC[1000]


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